Practice Problem

Developmental changes in ifenprodil binding within a brain region involved in vocal learning

This experiment evaluated the effects of age and sex on the expression of NMDA receptors within a brain region (LMAN) that is necessary for normal song learning in songbirds. The experiment involved zebra finches, a songbird in which only males produce learned song. Juvenile male zebra finches normally imitate only songs heard during a sensitive period that occurs between 20 and 55 days after hatching. The hypothesis being tested was whether the sensitive period for song learning correlates with age-related changes in the expression of NMDA receptors.

NMDA receptors play a central role in synaptic and behavioral plasticity, and their activation is necessary for normal avian song learning. Native NMDA receptors consist of several different subunits, and in vertebrate forebrain regions, a developmental decrease in the propensity for NMDA receptor-mediated plasticity is associated with a reduction in NR2B subunit expression. Here, we tested whether there are age-related changes in the expression of the NR2B subunit of the NMDA receptor within the LMAN of male and female zebra finches. To assess NR2B expression, we measured specific binding of ifenprodil, a ligand that binds only to NMDA receptors that contain the 2B subunit.

Animal ID

Specific Binding (pmol/mg tissue)

20d Female

AA86

0.298

AA95

0.263

AA89

0.167

AA91

0.230

Mean

0.239

SD

0.056

Animal ID

Specific Binding (pmol/mg tissue)

20d Male

AA87

0.594

AA94

0.416

AA88

0.566

AA90

0.521

Mean

0.524

SD

0.078

Animal ID

Specific Binding (pmol/mg tissue)

55d Female

AA21

0.198

NB1

0.188

AA32

0.223

AA30

0.233

Mean

0.211

SD

0.021

Animal ID

Specific Binding (pmol/mg tissue)

55d Male

AA22

0.229

NB3

0.246

AA31

0.219

AA29

0.276

Mean

0.243

SD

0.025

Click here for results

StatsWiki: nsc203practice3 (last edited 2012-01-20 21:44:12 by cpe-69-207-83-233)

MoinMoin Appliance - Powered by TurnKey Linux